Infection Density Dynamics of the Citrus Greening Bacterium “Candidatus Liberibacter asiaticus” in Field Populations of the Psyllid Diaphorina citri and Its Relevance to the Efficiency of Pathogen Transmission to Citrus Plants
Identifieur interne : 000579 ( Main/Exploration ); précédent : 000578; suivant : 000580Infection Density Dynamics of the Citrus Greening Bacterium “Candidatus Liberibacter asiaticus” in Field Populations of the Psyllid Diaphorina citri and Its Relevance to the Efficiency of Pathogen Transmission to Citrus Plants
Auteurs : Rie Ukuda-Hosokawa [Japon] ; Yasutsune Sadoyama [Japon] ; Misaki Kishaba [Japon] ; Takashi Kuriwada [Japon] ; Hisashi Anbutsu [Japon] ; Takema Fukatsu [Japon]Source :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2015.
Abstract
Huanglongbing, or citrus greening, is a devastating disease of citrus plants recently spreading worldwide, which is caused by an uncultivable bacterial pathogen, “
Url:
DOI: 10.1128/AEM.00707-15
PubMed: 25819961
PubMed Central: 4421049
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Infection Density Dynamics of the Citrus Greening Bacterium “<named-content content-type="genus-species">Candidatus</named-content> Liberibacter asiaticus” in Field Populations of the Psyllid <named-content content-type="genus-species">Diaphorina citri</named-content> and Its Relevance to the Efficiency of Pathogen Transmission to Citrus Plants</title><author><name sortKey="Ukuda Hosokawa, Rie" sort="Ukuda Hosokawa, Rie" uniqKey="Ukuda Hosokawa R" first="Rie" last="Ukuda-Hosokawa">Rie Ukuda-Hosokawa</name><affiliation wicri:level="1"><nlm:aff id="aff1">Okinawa Prefectural Plant Protection Center, Yaeyama Branch Office, Ishigaki, Okinawa, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okinawa Prefectural Plant Protection Center, Yaeyama Branch Office, Ishigaki, Okinawa</wicri:regionArea><wicri:noRegion>Okinawa</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Okinawa Prefectural Agricultural Research Center, Itoman, Okinawa, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okinawa Prefectural Agricultural Research Center, Itoman, Okinawa</wicri:regionArea><wicri:noRegion>Okinawa</wicri:noRegion></affiliation></author><author><name sortKey="Sadoyama, Yasutsune" sort="Sadoyama, Yasutsune" uniqKey="Sadoyama Y" first="Yasutsune" last="Sadoyama">Yasutsune Sadoyama</name><affiliation wicri:level="1"><nlm:aff id="aff3">Okinawa Prefectural Plant Protection Center, Naha, Okinawa, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okinawa Prefectural Plant Protection Center, Naha, Okinawa</wicri:regionArea><wicri:noRegion>Okinawa</wicri:noRegion></affiliation></author><author><name sortKey="Kishaba, Misaki" sort="Kishaba, Misaki" uniqKey="Kishaba M" first="Misaki" last="Kishaba">Misaki Kishaba</name><affiliation wicri:level="1"><nlm:aff id="aff4">Okinawa Prefectural Yaeyama Agriculture, Forestry and Fisheries 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Hosokawa, Rie" uniqKey="Ukuda Hosokawa R" first="Rie" last="Ukuda-Hosokawa">Rie Ukuda-Hosokawa</name><affiliation wicri:level="1"><nlm:aff id="aff1">Okinawa Prefectural Plant Protection Center, Yaeyama Branch Office, Ishigaki, Okinawa, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okinawa Prefectural Plant Protection Center, Yaeyama Branch Office, Ishigaki, Okinawa</wicri:regionArea><wicri:noRegion>Okinawa</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Okinawa Prefectural Agricultural Research Center, Itoman, Okinawa, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okinawa Prefectural Agricultural Research Center, Itoman, Okinawa</wicri:regionArea><wicri:noRegion>Okinawa</wicri:noRegion></affiliation></author><author><name sortKey="Sadoyama, Yasutsune" sort="Sadoyama, Yasutsune" uniqKey="Sadoyama Y" first="Yasutsune" last="Sadoyama">Yasutsune Sadoyama</name><affiliation wicri:level="1"><nlm:aff id="aff3">Okinawa Prefectural Plant Protection Center, Naha, Okinawa, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okinawa Prefectural Plant Protection Center, Naha, Okinawa</wicri:regionArea><wicri:noRegion>Okinawa</wicri:noRegion></affiliation></author><author><name sortKey="Kishaba, Misaki" sort="Kishaba, Misaki" uniqKey="Kishaba M" first="Misaki" last="Kishaba">Misaki Kishaba</name><affiliation wicri:level="1"><nlm:aff id="aff4">Okinawa Prefectural Yaeyama Agriculture, Forestry and Fisheries Promotion Center, Ishigaki, Okinawa, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okinawa Prefectural Yaeyama Agriculture, Forestry and Fisheries Promotion Center, Ishigaki, Okinawa</wicri:regionArea><wicri:noRegion>Okinawa</wicri:noRegion></affiliation></author><author><name sortKey="Kuriwada, Takashi" sort="Kuriwada, Takashi" uniqKey="Kuriwada T" first="Takashi" last="Kuriwada">Takashi Kuriwada</name><affiliation wicri:level="1"><nlm:aff id="aff5">Kagoshima University, Faculty of Education, Laboratory of Zoology, Kagoshima, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Kagoshima University, Faculty of Education, Laboratory of Zoology, Kagoshima</wicri:regionArea><wicri:noRegion>Kagoshima</wicri:noRegion></affiliation></author><author><name sortKey="Anbutsu, Hisashi" sort="Anbutsu, Hisashi" uniqKey="Anbutsu H" first="Hisashi" last="Anbutsu">Hisashi Anbutsu</name><affiliation wicri:level="1"><nlm:aff id="aff6">National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba</wicri:regionArea><wicri:noRegion>Tsukuba</wicri:noRegion></affiliation></author><author><name sortKey="Fukatsu, Takema" sort="Fukatsu, Takema" uniqKey="Fukatsu T" first="Takema" last="Fukatsu">Takema Fukatsu</name><affiliation wicri:level="1"><nlm:aff id="aff6">National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba</wicri:regionArea><wicri:noRegion>Tsukuba</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff7">Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff8">Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba</wicri:regionArea><wicri:noRegion>Tsukuba</wicri:noRegion></affiliation></author></analytic><series><title level="j">Applied and Environmental Microbiology</title><idno type="ISSN">0099-2240</idno><idno type="eISSN">1098-5336</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Huanglongbing, or citrus greening, is a devastating disease of citrus plants recently spreading worldwide, which is caused by an uncultivable bacterial pathogen, “<named-content content-type="genus-species">Candidatus</named-content> Liberibacter asiaticus,” and vectored by a phloem-sucking insect, <named-content content-type="genus-species">Diaphorina citri</named-content>. We investigated the infection density dynamics of “<italic>Ca</italic>. Liberibacter asiaticus” in field populations of <named-content content-type="genus-species">D. citri</named-content> with experiments using field-collected insects to address how “<italic>Ca</italic>. Liberibacter asiaticus” infection density in the vector insect is relevant to pathogen transmission to citrus plants. Of 500 insects continuously collected from “<italic>Ca</italic>. Liberibacter asiaticus”-infected citrus trees with pathological symptoms in the spring and autumn of 2009, 497 (99.4%) were “<italic>Ca</italic>. Liberibacter asiaticus” positive. The infections were systemic across head-thorax and abdomen, ranging from 10<sup>3</sup> to 10<sup>7</sup> bacteria per insect. In spring, the infection densities were low in March, at ∼10<sup>3</sup> bacteria per insect, increasing up to 10<sup>6</sup> to 10<sup>7</sup> bacteria per insect in April and May, and decreasing to 10<sup>5</sup> to 10<sup>6</sup> bacteria per insect in late May, whereas the infection densities were constantly ∼10<sup>6</sup> to 10<sup>7</sup> bacteria per insect in autumn. Statistical analysis suggested that several factors, such as insect sex, host trees, and collection dates, may be correlated with “<italic>Ca</italic>. Liberibacter asiaticus” infection densities in field <named-content content-type="genus-species">D. citri</named-content> populations. Inoculation experiments with citrus seedlings using field-collected “<italic>Ca</italic>. Liberibacter asiaticus”-infected insects suggested that (i) “<italic>Ca</italic>. Liberibacter asiaticus”-transmitting insects tend to exhibit higher infection densities than do nontransmitting insects, (ii) a threshold level (∼10<sup>6</sup> bacteria per insect) of “<italic>Ca</italic>. Liberibacter asiaticus” density in <named-content content-type="genus-species">D. citri</named-content> is required for successful transmission to citrus plants, and (iii) <named-content content-type="genus-species">D. citri</named-content> attaining the threshold infection level transmits “<italic>Ca</italic>. Liberibacter asiaticus” to citrus plants in a stochastic manner. These findings provide valuable insights into understanding, predicting, and controlling this notorious citrus pathogen.</p></div></front></TEI><affiliations><list><country><li>Japon</li></country><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><noRegion><name sortKey="Ukuda Hosokawa, Rie" sort="Ukuda Hosokawa, Rie" uniqKey="Ukuda Hosokawa R" first="Rie" last="Ukuda-Hosokawa">Rie Ukuda-Hosokawa</name></noRegion><name sortKey="Anbutsu, Hisashi" sort="Anbutsu, Hisashi" uniqKey="Anbutsu H" first="Hisashi" last="Anbutsu">Hisashi Anbutsu</name><name sortKey="Fukatsu, Takema" sort="Fukatsu, Takema" uniqKey="Fukatsu T" first="Takema" last="Fukatsu">Takema Fukatsu</name><name sortKey="Fukatsu, Takema" sort="Fukatsu, Takema" uniqKey="Fukatsu T" first="Takema" last="Fukatsu">Takema Fukatsu</name><name sortKey="Fukatsu, Takema" sort="Fukatsu, Takema" uniqKey="Fukatsu T" first="Takema" last="Fukatsu">Takema Fukatsu</name><name sortKey="Kishaba, Misaki" sort="Kishaba, Misaki" uniqKey="Kishaba M" first="Misaki" last="Kishaba">Misaki Kishaba</name><name sortKey="Kuriwada, Takashi" sort="Kuriwada, Takashi" uniqKey="Kuriwada T" first="Takashi" last="Kuriwada">Takashi Kuriwada</name><name sortKey="Sadoyama, Yasutsune" sort="Sadoyama, Yasutsune" uniqKey="Sadoyama Y" first="Yasutsune" last="Sadoyama">Yasutsune Sadoyama</name><name sortKey="Ukuda Hosokawa, Rie" sort="Ukuda Hosokawa, Rie" uniqKey="Ukuda Hosokawa R" first="Rie" last="Ukuda-Hosokawa">Rie Ukuda-Hosokawa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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